Reverse Transcriptase | Function, Structure & Application
Learn the reverse transcriptase enzyme definition. Explore the functions and structure of reverse transcriptase, and discover which organisms have this enzyme.
The Biotechnology Revolution: PCR and the Use of Reverse …
Jun 27, 1970 · At this point, the reverse transcriptase enzyme is added, and this enzyme proceeds to utilize the mRNA strand as a template for the synthesis of a complementary DNA strand.
The regulations of telomerase reverse transcriptase (TERT) in cancer
Jan 26, 2024 · Telomerase reverse transcriptase (TERT) is a key component of telomerase. Human TERT was first cloned in 1997 [5, 8, 9, 10], eight years after telomerase was first identified in HeLa …
The reverse transcriptase domain of prime editors contributes to DNA ...
Feb 17, 2025 · Reverse transcriptase (RT) has been shown to play a role in double-strand break repair in bacteria, yet the impact of the RT component of prime editors (PEs) on normal mammalian cellular …
Discovery and evolution of RNA and XNA reverse transcriptase function ...
Jul 20, 2020 · The ability of reverse transcriptases (RTs) to synthesize a complementary DNA from natural RNA and a range of unnatural xeno nucleic acid (XNA) template chemistries, underpins key …
Reverse transcription-quantitative PCR (RT-qPCR) without the need for ...
Jul 15, 2023 · For all samples, negative controls without reverse transcriptase enzyme were also prepared. Quantitative real-time PCR (qPCR) for satellite DNA expression analysis
Structural basis for pegRNA-guided reverse transcription by a ... - Nature
May 29, 2024 · The prime editor system composed of Streptococcus pyogenes Cas9 nickase (nSpCas9) and engineered Moloney murine leukaemia virus reverse transcriptase (M-MLV RT) collaborates with …
Protein-primed homopolymer synthesis by an antiviral reverse …
May 28, 2025 · A bacterial reverse transcriptase synthesizes polydeoxyadenylate to provide population-level immunity against viral infection.
Regulation of human telomerase in homeostasis and disease
Apr 2, 2020 · Telomere length is maintained by telomerase, which comprises a reverse transcriptase and a template RNA. Telomerase activity is disrupted in several genetic disorders, but is commonly …
Evolutionarily conserved role of telomerase reverse transcriptase in ...
Aug 6, 2025 · Akincilar, Fidan et al. report a conserved role for telomerase reverse transcriptase in regulating inflammation via the cGAS–STING nucleic acid-sensing pathway in myeloid cells, …